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OncoImmunin
®

Cellular Cytotoxicity


CyToxiLux® , GranToxiLux®, and PanToxiLux


Live, Single Cell-based Fluorogenic Cytotoxicity Assay Assay Kits

A tumor cell is engaged and then "killed" by a cytotoxic lymphocyte

Principle of assays:
Following the successful delivery of a lethal hit by cytotoxic lymphocytes, protease activities leading to cell death in individual target cells can be measured by flow cytometry or fluorescence microscopy. In each kit cleavage of a cell permeable fluorogenic substrate reports the following activities:

CyToxiLux® : downstream caspase
GranToxiLux®: granzyme B
PanToxiLux™: granzyme B and upstream caspase

 

Measurements with CyToxiLux®, GranToxiLux®, and PanToxiLuxTM are superior to bulk assays e.g., LDH and 51Cr release, in terms of both time (0.3-2.0 hr. vs. 4 hr.) and sensitivity (relatively weak CTL responses against subdominant epitopes are detectable).

Detailed CyToxiLux® PLUS! instructions are available for download in PDF format
Detailed GranToxiLux® PLUS! instructions are available for download in PDF format
Detailed PanToxiLux® instructions are available for download in PDF format
Detailed PanToxiLux™ P2D2 instructions are available for download in PDF format

NEW - prior to cytotoxicity assay, eliminate dead target cells with NFL1

onc1_pu-red.jpg (2403 bytes) OncoImmunin, Inc.
207A Perry Parkway, Suite 6
Gaithersburg, MD 20877
Phone: (301) 987-7881
FAX: (301) 987-7882
email:info@phiphilux.com
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