Metastasis detection is now possible using OncoImmunin's unique
fluorogenic substrates to assay the protease activities associated with metastasis of
malignant cells. Examples of current reagents for metastasis analysis are CaDiLux® for the
detection of cathepsin D and Elastolux® for the detection of elastase.
As with OncoImmunins caspase substrates,
these reporter molecules cross intact plasma membranes and are therefore able to detect
intracellular proteolytic activites in live cells. As an additional reagent for metastasis
detection, nonpermeable forms of the same fluorogenic substrates are being prepared for
assay of extracellular proteolytic activities.
In the near future, substrates for the matrix metalloproteases (MMPs)
as well as other proteases implicated in the metastatic process will become available.