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| The Objective: |
The Solution: |
 | Peptide substrates for proteases associated with apoptosis,
cell-mediated cytotoxicity, autophagy, metastasis, inflammation, and
viral infectivity. |
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A new class of cell and tissue
permeable fluorogenic substrates that have access to all environments of
live cells and tissues. |
| Diagnostics for apoptosis, cell-mediated cytotoxicity, autophagy,
metastasis, inflammation and viral infectivity. |
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| Cell and tissue permeable inhibitors for apoptosis, autophagy,
metastasis, inflammation and viral infectivity. |
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Modification
of OncoImmunin's design for cell and tissue permeable substrates:
substitution of a noncleavable bond at protease recognition sites |
| Drug delivery vehicles |
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Use of OncoImmunin's molecular
design which allows cell and tissue permeability |
| Introduction of oligonucleotides into live cells and animals,
especially humans, without causing damage, harm, or toxicity. |
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OncoImmunin, Inc. has designed,
synthesized, validated and patented a new class of molecules that can serve
as fluorogenic protease substrates for enzymatic activity determinations in
live cells as well as in serum, in vivo delivery vehicles for
oligonucleotides, e.g., siRNA, without toxicity, and resurrection or small
moleclues that have failed in vivo trials after exhibiting excellent
properties in solutionThe advantages of OncoImmunin's protease substrates over other
fluorogenic substrates are
4-fold:
| Fluorescent probes were selected to ensure accurate enzymatic activity
determination not only in solution, but in biological samples such as serum,
live cells, and tissues.
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| Intracellular protease activity can be visualized using a standard fluorescence
microscope- without any modification!
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| To ensure specificity, all substrates contain amino acid sequences from both
sides of cleavage sites, i.e. from Pn through Pn'.
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| Substrates assume loop conformations in solution (see computer modeled structure
under principles) which mimic active site loop conformations of
naturally occurring target molecules.
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OncoImmunin's fluorogenic protease substrate design allows determination of intracellular
enzymatic activity using a fluorescence microscope. Determination of activity in buffer
solution as well as in human serum can be accomplished using a standard fluorometer, or
for large sample throughput, a proprietary high-throughput screening (HTS)
system is available (ref the page)Note- Absorption measurements are also possible.
To learn more about OncoImmunin, Inc. and its enzymatic activity assay kits choose from a
category below:
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